Choose the correct answer from the alternatives given :
A DNA template plus primer with the structure
3 P - TG C GAATTAG C GACAT - P5
5 P - ATCGGTACGACGCTTAAC OH 3
(where P = a phosphate group) is placed in an in vitro DNA synthesis system containing $M g^{2 +}$ , an excess of the four deoxyribonucleoside triphosphates, etc. and a mutant form of E. Coli DNA polymerase I that lacks 5 $\to$ 3 exonuclease activity. The 5 $\to$ 3 polymerase and. 3 $\to$ 5 exonuclease activities of this aberrant enzyme are identical to those of normal E. Coli DNA polymerase I. It simply has no 5 $\to$ 3 exonuclease activity. What will be the structure of the final product?

Question

Choose the correct answer from the alternatives given :
A DNA template plus primer with the structure
3 P - TG C GAATTAG C GACAT - P5
5 P - ATCGGTACGACGCTTAAC OH 3
(where P = a phosphate group) is placed in an in vitro DNA synthesis system containing

M g^{2 +}

, an excess of the four deoxyribonucleoside triphosphates, etc. and a mutant form of E. Coli DNA polymerase I that lacks 5

\to

3 exonuclease activity. The 5

\to

3 polymerase and. 3

\to

5 exonuclease activities of this aberrant enzyme are identical to those of normal E. Coli DNA polymerase I. It simply has no 5

\to

3 exonuclease activity. What will be the structure of the final product?

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