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Biotechnology-Principles-and-Processes

You discovered a novel eukaryotic organism that glows in dark. You believe this trait is due to a single gene, and you wish to clone gene. which of following strategies is most likely to be successful?

Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into a plasmid Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into bacteria. Screen colonies for for the ability to glow in the dark. Isolate mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark. Isolate mRNA from the organism, reverse transcribe and genetate cDNA, Insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark.

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You discovered a novel eukaryotic organism that glows in dark. You believe this trait is due to a single gene, and you wish to clone gene. which of following strategies is most likely to be successful?

Isolate mRNA from the organism, reverse transcribe and genetate cDNA, Insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark.

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The Living World

Biological Classification

Plant Kingdom

Animal Kingdom

Morphology of Flowering Plants

Anatomy of Flowering Plants

Structural Organisations in Animals

Cell : The Unit of Life

Biomolecules

Cell Cycle and Cell Division

Transport in Plants

Mineral Nutrition

Photosynthesis in Higher Plants

Respiration in Plants

Plant Growth and Development

Digestion and Absorption

Breathing and Exchange of Gases

Body Fluids and Circulation

Excretory Products and Elimination

Locomotion and Movement

Neural Control and Coordination

Chemical Control and Coordination

Reproduction in Organisms

Sexual Reproduction in Flowering Plants

Human Reproduction

Reproductive Health

Principles of Inheritance and Variation

Molecular Basis of Inheritance

Evolution

Human Health and Disease

Strategies for Enhancement in Food Production

Microbes in Human Welfare

Biotechnology Principles and Processes

Biotechnology and its Applications

Organisms and Populations

Ecosystem

Biodiversity and Conservation

Environmental Issues

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Biotechnology Principles and Processes

Biology-MCQS

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