Chapter:

biotechnology-principles-and-processes

1. Taq polymerase which is used for amplification of DNA is related with

Hybridoma technique PCR technique Gene cloning rDNA technology

Hint Answer

2. A scientist is trying to engineer a plasmid for purposes of genetically modifying crops.
What is most appropriate first step in process?

Identify the gene of interest that will confer a benefit in the genetically modified crop. Cut both the plasmid and the gene with the same restriction enzyme. Use ligase to anneal the gene in the bacterial chromosome. Select the bacteriophage that will be used as a vector for gene delivery.

Hint Answer

3. Which is first cloned animal?

Dolly was first mammal to be cloned from an adult somatic cell, using process of nuclear transfer.
Dolly was cloned from a cell taken from mammary gland of a six-year-old Finn Dorset sheep and an egg cell taken from a Scottish Blackface sheep.
Electrical pulses were used to fuse mammary cell with an unfertilized egg cell, nucleus of which had been removed.
This fusion process resulted in transfer of mammary cell nucleus into egg cell, which n began to divide to form a complete embryo

So, correct answer is option C.

The restriction endonucleases are enzymes which cleave DNA at specific recognition sites present in DNA sequences. This helps in production of fragments and desired genes. This enzyme is widely used with respect to recombinant DNA technology.

Thus, correct answer is option A.

A phosphodiester bond occurs when exactly two of hydroxyl groups in phosphoric acid react with hydroxyl groups on or molecules to form two ester bonds. Phosphodiester bonds are central to all life on Earth as y make up backbone of strands of nucleic acid. DNAase deoxyribonuclease or simply nuclease is an enzyme which breaks down DNA by hydrolosis of phosphodiester bonds of its sugar-phosphate backbone. Enonuclease represents internal cleave which cleave phosphodiester bond internally.
So, correct option is option D

PCR - technique amplify DNA sample in three steps namely denaturation of target DNA, annealing and polymerization. Denaturation refers to rmal cycle that separates DNA strands and forms ssDNA. Annealing refers to pairing of primers to ssDNA and polymerisation is extension of primer into complete DNA strand complementary to template strand.

So, correct answer is 'Annealing'

A probe is a radioactively labelled DNA variable length (usually 100-1000 bases long) that can n be used in DNA samples to detect presence of nucleotide sequences ( DNA target) that are complementary to sequence in probe. The probes are used in DNA finger printing for identification of sample DNA fragments sorted by gel electrophoresis and sourn blotting. The radioactive probes are washed over nylon surface to allow ir joining to any DNA fragments of same composition reby matching different DNA samples. Disease specific probes are used in antenatal diagnosis of presence of even a single gene defect in foetus. Probes specific to pathogenic bacteria are used for detection of presence of se pathogens in individuals. Correct answer is D.

Biotechnology is broad area of biology involving living systems and organisms to develop or make products, or "any technological application that uses biological systems, living organisms, or derivatives reof, to make or modify products or processes for specific use" (UN Convention on Biological Diversity, Art. 2). Depending on tools and applications, it often overlaps with (related) fields of molecular biology, bio-engineering, biomedical engineering, biomanufacturing, molecular engineering, etc. For thousands of years, humankind has used biotechnology in agriculture, food production, and medicine. The term is largely believed to have been coined in 1919 by Hungarian engineer K�roly Ereky. In late 20th and early 21st centuries, biotechnology has expanded to include new and diverse sciences such as genomics, recombinant gene techniques, applied immunology, and development of pharmaceutical rapies and diagnostic tests.

So correct option is 'use of living organisms or substances obtained from m in industrial processes'.

Kary Mullis. Kary Banks Mullis (born December 28, 1944) is a Nobel Prize-winning American biochemist. In recognition of his invention of polymerase chain reaction (PCR) technique, he shared 1993 Nobel Prize in Chemistry with Michael Smith and earned Japan Prize in same year.

So, correct answer is 'Karry Mullis.'

The National Institute of Virology (NIV) is a premier virus research laboratory in India affiliated to University of Pune.

The National Institute of Communicable Diseases (NICD) is situated in New Delhi.

The Central Drug Research Institute is a multidisciplinary research laboratory in Lucknow, India, employing scientific personnel from various areas of biomedical sciences.

The National Institute of Nutrition (NIN) is an Indian Public health, Biotechnology and Translational research center located in Hyderabad, India.

Therefore, correct answer is option A.

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Chapter:

PCR technique

Identify the gene of interest that will confer a benefit in the genetically modified crop.

Dolly sheep

Cut DNA at specific base sequences

Endonuclease

Annealing

All of the above

Use of living organisms or substances obtained from them in industrial processes

Karry Mullis

National Institute of Virology - Pune

All Chapters

The Living World

Biological Classification

Plant Kingdom

Animal Kingdom

Morphology of Flowering Plants

Anatomy of Flowering Plants

Structural Organisations in Animals

Cell : The Unit of Life

Biomolecules

Cell Cycle and Cell Division

Transport in Plants

Mineral Nutrition

Photosynthesis in Higher Plants

Respiration in Plants

Plant Growth and Development

Digestion and Absorption

Breathing and Exchange of Gases

Body Fluids and Circulation

Excretory Products and Elimination

Locomotion and Movement

Neural Control and Coordination

Chemical Control and Coordination

Reproduction in Organisms

Sexual Reproduction in Flowering Plants

Human Reproduction

Reproductive Health

Principles of Inheritance and Variation

Molecular Basis of Inheritance

Evolution

Human Health and Disease

Strategies for Enhancement in Food Production

Microbes in Human Welfare

Biotechnology Principles and Processes

Biotechnology and its Applications

Organisms and Populations

Ecosystem

Biodiversity and Conservation

Environmental Issues

Topics

Biology mcqs

Taq polymerase which is used for amplification of DNA is related with

A scientist is trying to engineer a plasmid for purposes of genetically modifying crops. What is most appropriate first step in process?

Which is first cloned animal?

Restriction endonucleases are enzymes that are used by biotechnologists to

Which one of following hydrolyses internal phosphodiester bonds in a polynucleotide chain-

During PCR technique pairing of primers to ssDNA segment is called

DNA probe is used for

Which one of following approaches comes under category of biotechnology?

PCR or polymerase chain reaction was discovered by

Which one of following is correctly matched regarding an institute and its location?

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You need to login to ask any Questions from chapter biotechnology-principles-and-processes of biology-mcqs

A scientist is trying to engineer a plasmid for purposes of genetically modifying crops.
What is most appropriate first step in process?